相关实验视频

Updated: Jun 13, 2025

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Published on: December 22, 2020

在老化的膀中,先前存在的衰老性纤维细胞通过CXCL12分泌产生瘤宽容的利基

Satoru Meguro^1,2, Yoshikazu Johmura^3,4, Teh-Wei Wang¹

¹Division of Cancer Cell Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.

|September 9, 2024

概括

衰老通过增加分泌CXCL12的衰老纤维细胞促进膀癌. 消除这些细胞或阻断CXCL12信号显著抑制了小鼠模型中的瘤生长.

科学领域:

在瘤学瘤学.
老年学是指老年学的学科.
细胞生物学细胞生物学

背景情况:

衰老是癌症发展的重要危险因素.
将衰老与癌症风险增加联系在一起的机制尚未完全理解.
细胞衰老,以p16蛋白为特征,与衰老有关.

研究的目的:

调查与衰老相关的衰老纤维细胞在促进膀癌中的作用.
确定衰老细胞对致癌产生有助的分子机制.
探索针对膀癌老化的细胞的治疗策略.

主要方法:

利用了经过基因改造的小鼠膀癌模型.
采用单细胞RNA测序来分析老年小鼠的纤维细胞基因表达.
在体内研究了消除p16高衰老细胞和抑制CXCL12信号传递对瘤生长的影响.

主要成果:

在老鼠膀中,p16高衰老纤维细胞 (p16h-sn) 随着年龄的增长而积累.
p16h-sn纤维细胞作为炎症性癌症相关纤维细胞 (CAF) 起作用,促进瘤生长.
单细胞RNA测序在p16h-sn纤维细胞中发现了更高的CXCL12表达.
消除p16h-sn细胞或抑制CXCL12抑制了膀瘤的生长.
鉴定了一种p16h-sn CAFs的基因特征 (SMOC2,GUCY1A1,CXCL12,CRISPLD2,GAS1,LUM),与人类膀癌患者的预后不佳有关.

结论:

在老化的膀中,高衰老的纤维细胞形成了一个亲瘤的微环境.
这些衰老的纤维细胞主要通过CXCL12分泌促进膀瘤的生长.
针对衰老细胞和CXCL12信号传递,代表了与年龄相关的膀癌的潜在治疗策略.

更多相关视频

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

Published on: September 20, 2016

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...