通过LC-MS-MS对57种药物的血液和尿液的多类分析,以补充全面的DFC,DUID和死后检测
Luke N Rodda1,2, Megan Farley1, Steven Towler1
1Forensic Laboratory Division, Office of the Chief Medical Examiner, 1 Newhall St., San Francisco, CA 94124, United States.
Journal of analytical toxicology
|September 10, 2024
概括
一种新的LC-MS-MS方法简化了对药物促成犯罪 (DFC),尸体解剖 (PM) 调查和驾驶在药物影响下 (DUID) 测试的分析. 这种统一的方法通过检测法医样本中更广泛的药物,提高了实验室的效率.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 当前的法医药物测试往往需要多种方法,降低效率.
- 毒品辅助犯罪 (DFCs),尸体解剖 (PM) 调查和驾驶在毒品影响下 (DUID) 测试具有不同的分析需求.
- 标准化和高效的方法对于准确的法医案例工作至关重要.
研究的目的:
- 开发和验证一个简化的LC-MS-MS方法,用于在法医毒理学中进行全面的药物分析.
- 将DFC,PM和DUID测试的分析整合到一个单一,高效的工作流中.
- 为了满足法医药物检测的严格灵敏度要求.
主要方法:
- 开发了一种使用蛋白质沉和过提取的液态染色体-并联质谱法 (LC-MS-MS) 方法.
- 分析了57种不同类别的向药物和代谢物分析物,包括抗抑郁药,抗精神病药和幻觉剂.
- 根据学院标准委员会/美国国家标准协会 (ASB/ANSI) 的指导方针验证了该方法.
主要成果:
- 在不到6分钟的时间内从血液和尿液样本中达到57个分析物的化.
- 已确定的检测极限适用于敏感的DFC分析,也适用于PM和DUID病例.
- 成功地将该方法应用于超过9000个法医样本,识别了超过11000个阳性分析物.
结论:
- 开发的LC-MS-MS方法为分析法医毒理学中广泛的药物提供了一种统一而有效的方法.
- 这种综合测试策略提高了实验室的效率,并改善了在DFC,PM和DUID情况下检测物质.
- 该方法符合ASB/ANSI标准,为现代法医案例工作提供了强大的工具.
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