使用网络药理学确定了芝麻的抗低氧机制
Dan Song1, Mengjie Wang1,2, Yiyi Zhang1
1Joint Laboratory for Research on Active Components and Pharmacological Mechanism of Tibetan Materia Medica of Tibetan Medical Research Center of Tibet, School of Medicine, Xizang Minzu University, Xianyang, China.
来自Phlomis younghusbandii的芝麻胺,通过调节参与新陈代谢和炎症的点来对抗缺氧. 它特别与阿尔多-基因还原酶1B1 (AR) 相互作用,这对其抗低氧作用至关重要.
科学领域:
- 药理学和生物化学 药理学和生物化学
- 网络药理学网络药理学
- 分子生物学分子生物学
背景情况:
- 芝麻胺是Phlomis younghusbandii Mukerjee的活性成分,具有潜在的抗氧化和抗糖化特性.
- 缺氧是一种缺氧的情况,与各种疾病有关,需要探索新的治疗机制.
研究的目的:
- 通过网络药理方法阐明芝萨莫西德的抗低氧机制.
- 为了确定关键的分子标和途径,涉及到芝麻的治疗作用对抗缺氧.
主要方法:
- 网络药理学分析集成数据库 (GeneCards,OMIM,STRING,Metascape) 以确定与缺氧相关的目标和途径.
- 进行分子对接模拟,以评估芝沙胺对确定关键标的结合亲和力.
- 使用基于细胞的测试 (CCK-8,qPCR,Western Blot,ELISA) 进行体外验证,以确认芝麻胺对标表达和细胞反应的影响.
主要成果:
- 芝麻胺与与葡萄糖/脂质代谢,核酸代谢和炎症相关的多个点相互作用.
- 艾尔多基托减少酶1B1 (AKR1B1或AR) 被确定为芝麻胺抗低氧活性的关键标,具有有利的分子对接结果.
- 细胞实验证实,芝麻治疗显著改善AR表达,并在缺氧条件下调节炎症性细胞因子.
结论:
- 芝麻胺具有显著的抗低氧性质,通过复杂的分子点网络进行介导,特别是涉及AR.
- 这项研究提供了对芝麻胺抗低氧机制的全面了解,为其在低氧疾病中的潜在临床应用奠定了理论和实验基础.
更多相关视频
07:58Behavioral and Network Pharmacology-Based Analyses for the Traditional Mongolian Medicine Zadi-5 in a Rat Model of Depression
Published on: February 24, 2023
11:06Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia
Published on: April 7, 2023
相关概念视频
Structure-Activity Relationships and Drug Design
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
Sedatives and Hypnotics Drugs: Miscellaneous Agents
Melatonin congeners like ramelteon (Rozerem) and tasimelteon (Hetlioz) selectively bind to melatonin receptors (MT1 and MT2) and thus mimic the actions of melatonin, a hormone that regulates sleep-wake cycles. Tasimelteon is primarily used for non-24-hour sleep-wake disorder, common in blind patients. They are also used to treat conditions like insomnia...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Desensitization and Tachyphylaxis
Adrenergic Agonists: Indirect-Acting Agents
One mechanism involves depleting stored catecholamines by displacing them from synaptic vesicles. These agents, known as "displacers," are transported into vesicles at the expense of noradrenaline. Examples include amphetamine and tyramine, which lack a catechol moiety, resulting in prolonged action, improved oral...
