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相关概念视频

Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

5.9K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
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Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.5K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.5K
Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

2.0K
Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
273
Protein Transport to the Inner Chloroplast Membrane01:18

Protein Transport to the Inner Chloroplast Membrane

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Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

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Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
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Disruption of Polycystin Ciliary Localization and Channel Function by Autosomal Dominant Polycystic Kidney Disease-Causing Polycystin-1 Variants.

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相关实验视频

Updated: Jun 13, 2025

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
10:02

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Published on: December 12, 2014

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Inpp5e对于光受体外部段维护至关重要.

Mohona Gupta, Tylor R Lewis, Michael W Stuck

    bioRxiv : the preprint server for biology
    |September 10, 2024
    PubMed
    概括

    伊诺西多-5-酸酶e (INPP5E) 对光受体健康至关重要. 它的损失导致严重的外部细分缺陷和细胞死亡,导致失明.

    科学领域:

    • 眼科医生 眼科 眼科
    • 细胞生物学 细胞生物学
    • 遗传学 是一个遗传学.

    背景情况:

    • 伊诺西聚酸-5-酸酶e (INPP5E) 的突变与人类视网膜退化有关,包括乔伯特综合征和MORM综合征以及非综合征性失明.
    • 在小鼠模型中,INPP5E缺乏导致各种异常和受光受体外段形成障碍.

    研究的目的:

    • 调查Inpp5e在光受体外段开发和维护中的特定作用.
    • 阐明了Inpp5e依赖光受体完整性的细胞机制.

    主要方法:

    • 在光受体中为Inpp5e生成条件和可诱导的小鼠淘汰.
    • 分析光受体形态,外部细分结构,戈尔吉装置,蛋白质定位和actin网络动态.
    • 评估细胞外囊泡的形成和磁盘形态发生.

    主要成果:

    • 在光受体中失去Inpp5e,无论时间如何,都会导致严重的外部细分缺陷和光受体细胞损失.
    • 关键缺陷包括外部段缩短,减少新的圆盘形成,戈尔吉异常,罗多普辛错位化和细胞外囊泡积累.
    • 在淘汰细胞中观察到减少的活性蛋白网络和异常的膜旋转形成.

    结论:

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    Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
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    Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

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    Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors
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    Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
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    Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

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  • Inpp5e对于保持光受体外段结构和功能至关重要.
  • 该酶在光受体外段的持续更新过程中发挥着关键作用.
  • Inpp5e的功能障碍破坏了正常的磁盘形态发生,并导致光受体退化.