被定义的微生物群落OMM19.1定居的小鼠对C.敏感. 在没有先前的抗生素治疗的情况下,困难感染
bioRxiv : the preprint server for biology
|September 10, 2024
概括
合成的Oligo小鼠微生物群 19.1 (OMM19.1) 没有防止小鼠感染Clostridioides difficile. 需要额外的细菌来恢复肠道微生物群中的殖民抵抗力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 肠道微生物群可以防止病原体的殖民.
- 抗生素诱导的失生症增加了对C的敏感性. 困难的. 困难的.
- 奥利戈小鼠微生物群 19.1 (OMM19.1) 合成社区模仿了特定的无病原体小鼠肠道微生物群.
研究的目的:
- 评估OMM19.1殖民小鼠对C.的易感性. 在没有先前的抗生素治疗的情况下感染困难.
- 为了确定不同感染剂量的C.I.C.的影响. 在OMM19.1殖民小鼠上.
- 为了确定肠道微生物组成的变化,以下C. 在OMM19.1殖民小鼠中感染困难.
主要方法:
- 鼠标被OMM19.1.1殖民.
- 试验小鼠使用不同剂量的C. 困难的子 (103, 105, 107). 这种子通常会产生
- 分析了临床评分和肠道微生物组成.
主要成果:
- 被OMM19.1殖民的小鼠对C.敏感. 在所有测试剂量中都出现了困难感染.
- 临床评分与C.正相关. 难以使用的剂量.
- 在C.I.C.中, 困难感染导致Ligilactobacillus murinus和Escherichia coli的增加,并减少了Bacteroides caecimuris,Akkermansia muciniphila,Extibacter muris和Turicimonas muris的发生.
结论:
- 单独的OMM19.1合成群体并没有赋予对C.的殖民抵抗力. 困难的. 困难的.
- 需要额外的细菌物种来恢复保护性免疫力.
- 这突显了肠道微生物组在防止病原体入侵方面的复杂性.
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