相关实验视频

Updated: Jun 13, 2025

Analysis of Epididymal Protein Synthesis and Secretion

Analysis of Epididymal Protein Synthesis and Secretion

Published on: August 25, 2018

流行病特定的RNase A家族基因调节生育能力和小RNA处理

Joshua F Shaffer¹, Alka Gupta¹, Geetika Kharkwal¹

¹Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California, 95064.

bioRxiv : the preprint server for biology

|September 10, 2024

概括

四个流行病特异性基因调节男性生育能力和精子RNA处理. 删除这些基因 (Rnase9-12) 导致男性不育,改变了精子tRNA片段 (tRFs),影响了生育能力.

科学领域:

生殖生物学生殖生物学
在RNA生物学,RNA生物学.
遗传学遗传学是一个

背景情况:

精子小RNA,包括tRNA片段 (tRFs),对于父环境效应的传播至关重要.
在男性生殖道中产生tRFs的特定酶是未知的.
血管新生素是一种RNase A酶,在细胞应激下产生tRF.

研究的目的:

为了研究四种流行病特异性血管新生素对应物 (Rnase9,Rnase10,Rnase11,Rnase12) 的功能.
确定这些基因在男性生育能力和精子中的RNA处理中的作用.

主要方法:

产生了缺少所有四个Rnase基因 (Rnase9-12-/-) 的淘汰赛 (KO) 老鼠.
在KO小鼠中通过体外和体内受精试验评估雄性生育能力.
对小RNA水平 (tRFs,rsRNAs) 在KO小鼠的脑膜,光液和精子中的分析.

主要成果:

KO小鼠表现出完全的男性不育,未能在体内有效地受精卵细胞.
在KO epididymis和光液中观察到降低了tRF和rRNA衍生的小RNA (rsRNA) 的水平.
KO精子显示tRF水平显著降低,表明Rnase9-12在精子RNA组成中的作用.

结论:

四个特异性基因 (Rnase9-12) 对于男性生育和适当的RNA处理至关重要.
这些非正规的RNase A基因调节男性生殖道中tRFs和rsRNAs的生物发生和/或稳定性.
Rnase9-12在建立成熟精子小RNA配置文件方面发挥着关键作用,影响生育能力.

更多相关视频

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...