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大型动物模型物种在多能干细胞疗法研究和开发的视网膜疾病:一个系统的审查

Julia-Sophia Bellingrath¹, Kang V Li², Kanza Aziz³

¹Nuffield Laboratory of Ophthalmology, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom.

Frontiers in ophthalmology

|September 10, 2024

概括

大型动物模型 (LAM) 对于开发视网膜细胞疗法至关重要. 单层移植显示出比细胞悬浮体更好的整合,自主干细胞可能会减少在视网膜高级治疗药物 (ATMPs) 研究中的免疫排斥.

科学领域:

眼科医生眼科眼科
再生医学是一种再生医学.
翻译科学翻译科学

背景情况:

先进疗法药物 (ATMP) 在视网膜疾病方面表现有前途.
大型动物模型 (LAM) 对于将视网膜ATMP转化为临床用途至关重要.
在预算和基础设施方面,LAM研究面临着挑战.

研究的目的:

系统地评估物种分布,科学实用性和用于多能视网膜细胞治疗研究的LAM的方法.
帮助比较视网膜ATMP发育的不同LAM.

主要方法:

在PubMed,Embase,Science Direct和Google Scholar (2023年2月) 中进行系统的文献搜索.
关键词包括视网膜,干细胞,移植,大型动物,猪,子,狗和非人类灵长类动物.
包括22项关于非人类灵长类动物,猪,狗和子多能干细胞的研究.

主要成果:

单层移植显示出比细胞悬浮更高的网膜内合并.
免疫反应是异种移植的一个挑战.
自主诱导多能干细胞 (iPSC) 衍生的移植可能是必要的,以尽量减少排斥.

结论:

适当的LAM选择促进了视网膜ATMP的发展.
由于与人类眼睛的解剖学相似性,LAMs使临床相关的外科手术技术成为可能.
对于细胞/组织替代研究来指导临床试验方案,LAM测试仍然至关重要,尽管药理学研究的替代方法.

关键词:

免疫抑制是一种免疫抑制.临床前 (体内) 研究.视网膜退化视网膜退化干细胞移植干细胞移植手术技巧手术技巧手术技巧

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