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使用FragPipe-Analyst进行定量蛋白质学数据的分析和可视化.

Yi Hsiao1, Haijian Zhang2, Ginny Xiaohe Li3

  • 1Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Michigan 48109, United States.

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概括
此摘要是机器生成的。

FragPipe-Analyst是一个新的R闪亮的Web服务器和R包,为FragPipe生成的定量蛋白质组学数据提供用户友好的下游统计分析和可视化. 它支持各种量化工作流程,并提供高级功能,用于增强数据解释.

关键词:
在AP-MS中使用AP-MS.在FragPipe中,可以使用FragPipe.这是一个 LiP-MS 系统.生物信息学是一种生物信息学.下游分析下游分析这是开源的,开源的.蛋白质的酸化是蛋白质的酸化.软件 软件 软件 软件 软件

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • FragPipe是一个流行的计算蛋白质组学平台,以速度和可用性而闻名.
  • 现有的工作流需要单独的工具,用于下游统计分析和定量蛋白质组学数据的可视化.

研究的目的:

  • 为FragPipe用户开发一个易于使用的下游分析和可视化工具.
  • 为定量蛋白质组学数据提供全面的统计分析和可视化能力.

主要方法:

  • 开发了FragPipe-Analyst,一个R光泽的Web服务器.
  • 创建了FragPipeAnalystR,这是一个扩展功能的R包.
  • 支持无标签量化,双重质量标签和数据独立获取工作流.
  • 实现了缺失值归算,质量控制,聚类,微分表达式分析 (Limma) 和丰富分析 (Enrichr).

主要成果:

  • FragPipe-Analyst提供了一个用户友好的界面,用于下游蛋白质组学数据分析.
  • 该工具支持主要的量化工作流程,并包括先进的统计方法.
  • FragPipeAnalystR包允许对翻译后修改 (PTM) 进行特定地点的分析.

结论:

  • FragPipe-Analyst和FragPipeAnalystR为全面的定量蛋白质组学数据分析提供了必要的工具.
  • 这些开源资源增强了FragPipe平台对研究界的实用性.
  • 这些工具促进了高级分析和定制可视化,包括PTM网站特定分析.