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在口腔链球菌中对结合性和染色体集成的移动遗传元素进行基因组分析

Erica Lee¹, Erin Priutt², Seth Woods²

¹New York Institute of Technology College of Osteopathic Medicine, Jonesboro, Arkansas, USA.

Applied and environmental microbiology

|September 10, 2024

概括

研究人员在口腔链球菌中探索了移动遗传元素,发现了可以转移基因的多种元素. 这增强了对这些细菌如何适应的理解,可能会影响口腔健康和疾病.

科学领域:

微生物学微生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

口腔链球菌是口腔微生物群的关键成员,影响健康和疾病.
水平基因转移,特别是通过结合,对于口腔中的细菌适应和进化至关重要.
有限的信息存在于口腔细菌的结合机制.

研究的目的:

在六种口腔链球菌物种中研究结合性和染色体集成的移动遗传元素 (cciMGEs) 的多样性.
描述已识别的 cciMGEs 的货物基因和放松酶类型.
评估cciMGE对口腔链球菌致病性和抗菌素耐药性的潜在影响.

主要方法:

使用现有工具对551个口腔链球菌基因组进行生物信息分析.
识别和分类ccMGE,包括整合和结合元件 (ICE) 和整合和可调动元件 (IME).
对 cciMGE 大小,货物基因,放松酶类型以及切除和循环化潜力的分析.

主要成果:

在6种口腔链球菌物种中确定了486种ccMGE,包括173种ICE和233种IME.
观察到cciMGE大小,载荷基因和放松酶类型的显著多样性,包括新的放松酶蛋白.
检测到一个广泛传播的IME携带多药物耐药性载体,以及在*Streptococcus mutans*中切除/循环化的证据.

结论:

在口腔链球菌中,cciMGEs是多种多样的和普遍存在的,显著地塑造了它们的遗传景观.
这些移动元素携带的基因可以影响细菌的生存,病原性和抗菌素耐药性.
了解cciMGEs提供了对口腔细菌影响人类健康的进化和适应的关键见解.

关键词:

抗微生物耐药性抗微生物耐药性结合方式结合方式横向基因转移是指水平基因转移.整合和结合元素的整合和结合元素.口腔微生物组是口腔的微生物组.口服链球菌口服链球菌毒性因素是毒性的因素.

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