时间解决的多组学揭示了沙门氏菌在饮食引起的炎症期间的多种代谢策略
Katherine Kokkinias1, Anice Sabag-Daigle2, Yongseok Kim3
1Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA.
mSphere
|September 10, 2024
概括
沙门氏菌Typhimurium将其新陈代谢适应饮食和感染阶段. 高脂肪饮食促进炎症和改变细菌基因表达,揭示了慢性沙门氏菌感染的潜在治疗点.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 代谢学 代谢学 代谢学
背景情况:
- 抗生素耐药性和慢性感染需要新的治疗策略.
- 饮食变化显著影响肠道病原体的存活率和毒性.
- 沙门氏菌Typhimurium的代谢适应饮食和感染时间表的理解很少.
研究的目的:
- 为了研究沙门氏菌Typhimurium对纤维性与高脂肪饮食的代谢反应,在老鼠模型中随着时间的推移.
- 识别与不同感染阶段相关的沙门氏菌基因表达模式.
- 为了发现沙门氏菌感染的潜在治疗点.
主要方法:
- 多omics方法 (基因表达,核糖体活动).
- 在吃纤维和高脂肪饮食的小鼠中对沙门氏菌Typhimurium进行比较分析.
- 在早期,高峰期和晚期感染阶段进行纵向采样.
主要成果:
- 沙门氏菌Typhimurium在高脂肪饮食中优先使用呼吸道电子受体,与宿主炎症相关.
- 对于每一个感染阶段,都确定了不同的基因表达特征 (呼吸道,碳,病原体).
- 宿主细胞进入的基因在整个感染过程中出乎意料地表达,这表明细菌亚群或应激反应.
结论:
- 沙门氏菌Typhimurium在对饮食和时间变化的反应中表现出显著的代谢可塑性.
- 确定了特定阶段的沙门氏菌基因,可以作为新的治疗点.
- 了解沙门氏菌的代谢调节对于开发针对抗生素耐药性感染的有针对性的干预措施至关重要.
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