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Updated: Jun 13, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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多个生物分析测定平台的比较,用于siRNA治疗药物的量化

Karan Agrawal1, Laurelle K Calliste1, Shaofei Ji2

  • 1Bioanalysis Discovery & Development Sciences, Janssen Research & Development, LLC, Spring House, PA 19477, USA.

Bioanalysis
|September 10, 2024
PubMed
概括

四种用于量化寡核酸治疗药物的生物分析方法显示了可比的结果. 混合LC-MS和SL-RT-qPCR提供了最高的灵敏度,而SL-RT-qPCR和HELISA为siRNA分析提供了最佳的吞吐量.

关键词:
这就是LC-MS.捕获探测器捕获探测器混合化 混合化 混合化带结合测定试验锁定核酸的核酸被锁定.氧核酸类的部分.短干扰RNA (siRNA) 是一种短干扰RNA.干循环逆转录定量PCR

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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