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在Dactylogyrus crucifer和Dactylogyrus zandti中,完整的线粒体基因组揭示了Dactylogyrus内部不同的编码子使用模式

Cui-Lan Hao¹, Yuan-Yuan Yang², Nian-Wen Wei²

¹College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals, China.

|September 10, 2024

概括

这项研究对两种Dactylogyrus物种的线粒体基因组 (线粒体基因组) 进行了测序和分析,将已知的Dactylogyrus线粒体基因组数据库扩展了50%以上,并有助于未来的遗传学研究.

科学领域:

寄生虫学的寄生虫学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

作为Dactylogyridae家族的一部分,Dactylogyrus属主要感染cyprinoid鱼的.
尽管大小庞大,但迄今为止只研究了三种线粒体基因组 (线粒体基因组).

研究的目的:

测序和描述Dactylogyrus crucifer和Dactylogyrus zandti的完整线粒体基因组.
将这些线粒基因组与其他单基因组进行比较,以了解基因组织,结构和密码体的使用.
为了为有限的Dactylogyrus线粒基因组数据库做出贡献,并协助家族遗传学分析.

主要方法:

整个线粒体基因组测序使用Illumina NovaSeq.
对基因结构,tRNA二次结构和相对同义码子使用的生物信息分析.
使用贝叶斯信息和最大概率方法构建家族遗传树.

主要成果:

测序了D. crucifer (14,403个bp) 和D. zandti (18,584个bp) 的线粒基因组.
确定了保存的基因组织和定位,与其他Dactylogyrus物种一致.
在Dactylogyridae中观察到独特的核酸组成,与其他Dactylogyridea相比,Dactylogyrus蛋白质编码基因中的A+T含量较低.
在Dactylogyrus中发现了非保存的和较低的相对同义符号的使用,与其他单体动物相比,在密切相关的物种中具有相同的模式.
遗传学分析表明,来自Leuciscinae亚家族的Dactylogyrus物种形成了一个独特的姐妹群.

结论:

该研究显著增加了Dactylogyrus的可用线粒基因组数据,超过50%的扩展.
这些发现为Dactylogyrus的线粒体特征提供了宝贵的见解,包括核酸组成和密码子使用偏差.
生成的数据将促进未来的研究线粒体基因进化和 Monogenea.com 内的遗传学分析.

关键词:

基础组成基础组成科登使用科登使用达克提洛吉鲁斯 (Dactylogyrus) 是一个名字.进化进化进化进化进化进化进化线粒体基因组是指线粒体的基因组.单一的单一的人类的家谱 (Phylogeny) 是一个学科.

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