使用具有或没有分解迹象的个体的骨样本进行分子年龄预测:一种多变量方法,结合了翻译后蛋白质修饰和DNA甲基化分析
J Becker1, V Bühren1, L Schmelzer2
1Institute of Legal Medicine, University Hospital Duesseldorf, 40225, Duesseldorf, Germany.
International journal of legal medicine
|September 10, 2024
概括
使用DNA甲基化,托西丁和D-酸等分子标记物的法医年龄预测显示出有希望. 结合这些方法可以提高准确性,特别是在腐烂的遗骸中,有助于对身份不明尸体的调查.
科学领域:
- 法医科学 法医科学 法医科学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 已故个体的年代年代估计对于识别未知尸体至关重要.
- 由于矿物化,骨提供保存的组织用于分子分析.
- 基因甲基化 (DNAm),托西丁 (Pen) 和D-酸 (D-Asp) 是潜在的年龄预测标记物.
研究的目的:
- 开发和评估使用DNAm,Pen和D-Asp的年龄预测模型.
- 评估将这些分子时钟结合起来用于年龄预测的好处.
- 为了研究这些标记物在分解的骨样本中的适用性.
主要方法:
- 分析了86名 (训练) 和92名 (测试) 个体的头骨样本.
- 在6个CpG位点测量了DNA甲基化,使用大规模并行测序.
- 使用高性能液体染色学量化Pen和D-Asp水平.
主要成果:
- 单个模型在未分解的样本中实现了平均绝对误差 (MAE) 的5.5年 (DNAm),7.7年 (Pen) 和11.7年 (D-Asp).
- 分解样本显示所有标记的精度降低 (MAE:7.4-11.8年).
- 结合标记物提高了分解样本的准确性,特别是异常值,而与未分解样本的DNAm单独可比.
结论:
- 基因甲基化,西丁和D-酸是可行的分子时钟,用于年龄预测.
- 将这些标记物结合起来可以提高年龄预测的准确性,特别是在具有挑战性的腐烂遗骸中.
- 这种多标志物方法对未识别个体的法医年龄估计具有重大潜力.
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