预分析程序可以提高周围假肢感染患者的微生物培养产量吗?
Juliane Käschner1, Christoph Theil2, Georg Gosheger1
1Department of General Orthopaedics and Tumor Orthopaedics, University Hospital Münster, Münster, Germany.
BMC microbiology
|September 10, 2024
概括
在硫糖基媒介中储存周围假肢关节感染 (PJI) 组织样本并没有增加整体培养产量,但减少了培养负结果,并发现了更多的生物体. 这会影响感染诊断和治疗策略.
科学领域:
- 微生物学 微生物学
- 整形外科手术 整形外科手术
- 传染性疾病 传染性疾病
背景情况:
- 精确的病原体检测对于诊断和治疗周围假肢关节感染 (PJI) 至关重要.
- 虽然分析方法已经进步,但样品处理的预分析程序需要进一步调查.
- 这项研究旨在比较四种预分析方法对培养产量的有效性.
研究的目的:
- 为了比较四种不同的预分析程序的培养产量,以诊断周围假肢关节感染 (PJI).
- 评估硫糖介质和储存温度对微生物检测的影响.
- 评估分析前方法对识别PJI引起病原体的影响.
主要方法:
- 一项涉及71名被诊断患有PJI的患者的横截面研究.
- 组织样本被分为四组:无菌容器 (A) 或室温4°C或37°C24小时的硫糖介质 (B-D).
- 培养物被监测成长,并记录和比较了阳性样本,生物体数量和阳性化时间.
主要成果:
- 硫糖介质没有显著改善整体培养产量,但减少了培养负结果 (10%与没有硫糖).
- 在13%的用硫糖酸盐培养的样本中发现了额外的生物.
- 超过第7天的生长仅在硫糖基中观察到,而4°C的储存导致了更长的时间来呈阳性.
结论:
- 在硫糖中进行分析前储存并没有提高PJI的整体培养产量,但减少了培养负的感染.
- 在采样过程中将硫糖介质纳入,有助于识别额外的生物.
- 标准的在无菌容器中进行预分析储存的总产量仍然相当,但硫糖在检测多微生物感染方面具有好处.
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