Fgf9促进切口牙上皮干细胞的存活和牙的形成
Lingyun Tang1, Mingmei Chen1, Min Wu2
1State Key Laboratory of Medical Genomics, Research Center for Experimental Medicine, Rui- Jin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Road II, Building #17, Shanghai, 200025, P.R. China.
Stem cell research & therapy
|September 10, 2024
概括
纤维细胞生长因子9 (FGF9) 对于牙上皮干细胞的存活和牙形成至关重要. FGF9通过表皮-介质细胞相互作用调节茎状,促进乳腺细胞分化,这对牙发育至关重要.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞因子在牙发育和牙组织工程中起着至关重要的作用.
- 纤维细胞生长因子9 (FGF9) 在牙发育过程中始终在牙上皮质组织中表达.
- 以前的研究表明,Fgf9淘汰赛小鼠没有明显的牙异常,这表明有补偿机制.
研究的目的:
- 调查FGF9在牙发育中的精确作用,使用主导负功能丧失突变 (Fgf9S99N).
- 阐明FGF9影响牙上皮干细胞 (DESC) 生存和牙形成的机制.
主要方法:
- 使用了一种新的Fgf9S99N小鼠模型与微型CT,组织形态分析和基因表达试验.
- 评估DESC生存和差异化,使用球体形成试验和牙扩展培养.
- 采用细胞/组织培养,基因表达分析,抑制剂和抗体阻塞来探索FGF9信号通路.
主要成果:
- Fgf9S99N突变导致乳腺细胞减少,质形成受损,宫环中亡增加.
- FGF9通过激活ERK/CREB信号来促进DESC的生存,而不会影响扩散.
- FGF9通过介质细胞依赖的机制刺激 amelogenesis,包括通过 MAPK/ERK 信号诱导 FGF3 和 FGF10 的分泌.
结论:
- FGF9对于牙上皮干细胞的存活和牙质形成至关重要.
- FGF9充当双向调节器,保持DESC干度,同时促进乳腺细胞分化.
- 由FGF9调解的表皮-介质相互作用对于正确的牙面膜发育至关重要.
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