快速识别由Glomerella cingulata产生的植物毒素使用基于高分辨率质谱的资格,有针对性的结构确认和其特征调查
Xin Yu1, Zhiyang Liu1, Huidi Zhang1
1College of Plant Health and Medicine, Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, Qingdao Agricultural University, Qingdao, People's Republic of China.
Journal of basic microbiology
|September 11, 2024
概括
这项研究确定了来自Glomerella cingulata的六种植物毒素,这是果Glomerella叶斑 (GLS) 的原因. 两种毒素,HMFCA和TAA,显示出显著的植物毒性,有助于理解和控制这种破坏性的果病.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 生物化学 生化学
背景情况:
- 在中国,Glomerella cingulata导致破坏性果Glomerella叶斑 (GLS).
- 植物毒素对疾病发展至关重要,但G. cingulata.之前没有发现任何植物毒素.
- 了解这些毒素是管理果GLS的关键.
研究的目的:
- 快速识别由G. cingulata.产生的植物毒素.
- 确认它们的结构并调查植物毒性.
- 提供有关果GLS病原和控制的见解.
主要方法:
- 使用UPLC-HRMS和GC-MS进行原始毒素样本的初步选.
- 使用国家图书馆和文献来识别潜在的植物毒性化合物.
- 通过使用参考标准进行HPLC-DAD,UPLC-MS/MS和GC-MS的目标结构验证.
- 进行了植物毒性测试,以评估已识别的化合物的作用.
主要成果:
- 确认了六种植物毒素:5-基甲基-2-碳酸 (HMFCA),2,5-bis(基甲基) (BHMF),2-酸 (FA),2,3-butanediol,跨酸 (TAA) 和 cis-aconitic 酸 (CAA).
- HMFCA和TAA显示出显著的植物毒性.
- 所有确定的毒素都是非宿主选择性的,并表现出协同的植物毒性.
- 所有测试的菌株通常都产生BHMF,HMFCA,FA,TAA和CAA.
- 高温或pH值抑制或消除了这些化合物的植物毒性.
结论:
- 解了导致果GLS的主要植物毒素.
- 确定HMFCA和TAA作为关键的植物毒剂.
- 描述了毒素作为非宿主选择性和协同作用.
- 证明了环境因素 (温度,pH) 对毒素活性的影响.
- 为开发控制果GLS的策略提供了基础知识.
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