含有 SET 域的蛋白质 MdSDG26 负面调节了果中的 Alternaria alternata 抵抗力
Wenyun Shen1, Dehui Zhang2, Zitong Zhang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Plant, cell & environment
|September 11, 2024
概括
这项研究确定了果中的MdSDG26基因,该基因通过调节组织蛋白甲基化来增强对Alternaria alternata叶斑病的抵抗力. 这一发现为对抗真菌病原体的植物防御机制提供了新的见解.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 由Alternaria alternata引起的果叶斑是果生产的重大威胁.
- SET域组 (SDG) 蛋白质是参与植物发育和应激反应的基因组甲基转移酶,但它们在病原体耐药性中的作用尚不清楚.
研究的目的:
- 调查病原体诱导基因MdSDG26在果 (Malus × domestica) 抗 Alternaria alternata 的作用.
- 阐明MdSDG26赋予耐药性的分子机制,重点关注其在组织素甲基化中的功能.
主要方法:
- 基因克隆和MdSDG26及其转录变体的特征.
- 过时的过度表达和敲击实验来评估MdSDG26的功能.
- 使用ChIP-qPCR进行的基因组甲基化分析 (H3K4me3,H3K36me3).
- 转录组分析 (RNA-Seq) 来识别MdSDG26调节的基因.
- 作为下游目标的MdNTL9的功能分析.
主要成果:
- MdSDG26被确定为果中的病原体诱导基因,具有两个功能转录变异.
- 过度表达MdSDG26增加了全球H3K4me3和H3K36me3水平,而淘汰减少了H3K36me3.3.
- 在A. alternata感染后,MdSDG26显著改变了果转录组.
- MdSDG26 调节 H3K36me3 和 H3K4me3 在 MdNTL9 的促进子和外子区域,这是阻力的负调节器.
- MdNTL9在MdSDG26介导的对A. alternata.耐药性中发挥着至关重要的作用.
结论:
- 果中MdSDG26作为抗Alternaria alternata叶斑的积极调节剂.
- MdSDG26通过调节组织素甲基化 (H3K36me3和H3K4me3) 来发挥其功能.
- MdSDG26-MdNTL9通路是赋予植物对真菌病原体免疫力的关键分子机制.
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