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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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使用共价有机框架的序列依赖单分子DNA传感纳米孔.

Linru Guo1, Xiao-Lei Xing1,2, Qiaobo Liao1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

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概括

研究人员开发了一种新的共价有机框架 (COF) 纳米孔,用于无酶单分子DNA测序. 这种固态纳米孔显示了区分DNA基和序列的前景,推进了纳米孔测序技术.

关键词:
在COF纳米孔中.单核酸 mononucleotide 的使用方法这是一个纳米管子.测序的测序是指测序的测序.模拟模拟是指一个模拟模拟器.一个单分子DNA的DNA.

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科学领域:

  • 纳米技术纳米技术
  • 分子生物学分子生物学
  • 材料科学 材料科学 材料科学

背景情况:

  • 使用纳米孔技术无酶单分子测序提供了广泛的应用,但面临着固态纳米孔的挑战.
  • 精确的单分子DNA测序需要精确控制纳米孔大小和合规性,这是传统固态纳米孔努力实现的.
  • 生物纳米孔适合无酶测序,但为了更好的控制和可扩展性,需要寻找固态替代品.

研究的目的:

  • 制造和描述一种新的固态纳米孔装置,用于无酶单分子DNA测序.
  • 评估开发的纳米孔区分DNA基和识别特定序列模式的能力.
  • 研究影响DNA分子转位和纳米孔内信号检测的因素.

主要方法:

  • 使用超薄的二维 (2D) 共价有机框架 (COF) 在石英纳米管尖上的纳米片制造纳米孔设备.
  • 对COF纳米孔大小 (≈1.1 nm) 和原子尺度结构一致性的表征.
  • 进行电气测量以检测来自单个DNA分子的电流阻塞,并执行有限元模拟.

主要成果:

  • 该COF纳米孔装置证明了大致区分四个DNA基 (dAMP,dCMP,dGMP,dTMP) 的能力.
  • 对于150个核酸模型DNA分子,观察到独特的序列特定的电阻电流模式,其中显著的百分比是独特的阻塞事件.
  • 有限元模拟证实了DNA当前封锁模式取决于纳米孔在纳米管内相对位置.

结论:

  • 协价有机框架 (COF) 纳米片使固态纳米孔的制造具有精确的尺寸,适合单分子DNA分析.
  • 开发的COF纳米孔装置通过检测依赖序列的电信号,显示了无酶单分子DNA测序的潜力.
  • 这项研究代表了通过固态纳米孔技术实现精确的单分子DNA测序的重大进展.