相关实验视频

Updated: Jun 13, 2025

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

Published on: August 19, 2016

揭示了Pasteurella multocida毒素对多个器官系统的致命影响

Jianlin Yuan¹, Jinfeng Li¹, Senyan Du^1,2,3

¹Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Frontiers in microbiology

|September 11, 2024

概括

菌多种毒素 (PMT) 严重损害了猪和小鼠的多个器官,特别是脏和肺. 这项研究详细介绍了PMT.

科学领域:

兽医病理学兽医病理学
微生物毒理学微生物毒理学
动物科学动物科学

背景情况:

D型的Pasteurella multocida在养猪业中造成重大经济损失.
虽然已知有缩性鼻炎,但PMT对其他猪器官的影响尚未完全理解.
在Pasteurella multocida D型感染中,PMT是关键的毒性因子.

研究的目的:

为了研究Pasteurella multocida毒素 (PMT) 对猪细胞的细胞毒性作用.
为了确定PMT在小鼠和猪中的体内毒性和位器官.
阐明PMT在各种器官中造成的病理损伤.

主要方法:

净化和表达的PMT.
在四个猪细胞系 (LLC-PK1,PAM,IPEC,ST) 的体外细胞毒性测定.
在小鼠和猪体内毒性研究,包括剂量反应和病理学检查.

主要成果:

在实验室中,LLC-PK1细胞对PMT表现出最高的敏感性.
在度分别为0.1μg/kg和0.5μg/kg的小鼠中,PMT诱导体重减轻和死亡.
在猪中,PMT对心脏,脏,肺部,肝脏和脏造成了显著的损伤,严重的脏和心脏病理.

结论:

PMT在体外和体内具有很高的毒性,影响多个器官系统.
脏和肺部特别容易受到PMT引起的损伤.
这些发现为进一步研究PMT的细胞毒性机制和对位器官的影响提供了基础.

关键词:

帕斯图雷拉多种毒素 (PMT) 的毒素动物杀伤性动物杀伤性细胞毒性细胞毒性在猪中具有毒性.器官损伤器官损伤器官损伤

更多相关视频

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Published on: July 12, 2018

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

相关概念视频

Types of Toxins

Types of Toxins

Humans continually engage with an environment rich in potentially harmful chemicals. These are introduced to our bodies through inhalation, ingestion, or skin contact. These chemicals exist in various forms, such as air and environmental pollutants, agricultural chemicals, organic solvents, and heavy metals.
Air pollutants, primarily gases, pose significant threats to respiratory health, leading to conditions like hypoxia, lung cancer, and in extreme cases, death.
Environmental pollutants like...

Local Anesthetics: Adverse Effects

Local Anesthetics: Adverse Effects

While local anesthetics are generally safe and well-tolerated, they can occasionally cause adverse effects that vary in severity. Local anesthetics can induce toxicity at two distinct levels. They can either produce local effects through direct contact with the neural elements or be absorbed into the bloodstream from the injection site, leading to systemic effects.
Once absorbed into the systemic circulation, local anesthetics can affect the organs that depend on the functioning of sodium...