多模式机械显微镜揭示了三维的乳腺癌球状体的机械表型
Alireza Mowla, Matt S Hepburn, Jiayue Li
1School of Human Sciences, The University of Western Australia, Perth, WA 6009, Australia.
APL bioengineering
|September 11, 2024
概括
癌细胞的入侵涉及细胞机制和瘤微环境. 新的3D成像揭示了转移性细胞变软和变形,使入侵成为可能.
科学领域:
- 生物物理学的生物物理.
- 癌症生物学 癌症生物学
- 机械生物学 机械生物学
背景情况:
- 癌细胞的入侵是复杂的,受细胞变形性和细胞外基质 (ECM) 的影响.
- 在3D瘤微环境 (TME) 中癌细胞扩散期间理解局部生物机械变化缺乏共识.
- 目前用于3DTME的机械量化工具仅限于表面测量,阻碍了3D分析.
研究的目的:
- 克服3DTME内癌细胞球体的局部力学量化的局限性.
- 利用多式机械显微镜进行癌细胞的3D机械造型.
- 在3D环境中研究细胞力学,ECM特性和癌细胞入侵之间的关系.
主要方法:
- 开发和应用多式联络机械显微镜.
- 集成的基于光学连贯显微镜的弹性成像与共聚焦光显微镜.
- 在3DTME中量化癌细胞球体的局部机械特性.
主要成果:
- 非转移性球状体在各种环境中没有入侵的情况下显示出外围细胞弹性增加.
- 转移性球体在硬的TME中表现出ECM介导的软化.
- 在软的TME中,转移性球状体发起了外围软化的入侵,与早期传播相关.
结论:
- 癌细胞的入侵是通过在3D环境中增加细胞变形性来促进的.
- 多模态机械显微镜能够在3D中进行定量,现场机械生物学.
- 癌细胞的局部机械变化是转移潜力的关键决定因素.
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