Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

8.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.6K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.4K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Membrane protein structure and dynamics probed by MicroED.

Biochemical Society transactions·2026
Same author

Direct from the seed: an atomic resolution protein structure by ab initio MicroED.

Nature communications·2026
Same author

Rapid Structural Analysis of Natural Products Using MicroED.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

β-barrels from short macrocyclic peptides.

Chemical communications (Cambridge, England)·2026
Same author

Discovery of Oxyacanthine Dihydrochloride Monohydrate Polymorphs from Obfuscated Samples by Microcrystal Electron Diffraction.

ChemMedChem·2025
Same author

pH-dependent regulation in SLC38A9.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: Jun 13, 2025

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

6.6K

能量过使得宏分子MicroED数据能够以亚原子分辨率进行过.

Max T B Clabbers1,2, Johan Hattne1,2, Michael W Martynowycz2

  • 1Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095.

bioRxiv : the preprint server for biology
|September 11, 2024
PubMed
概括

现在可以通过MicroED的电子计数和能量过实现高分辨率的宏分子结构. 这种技术提高了信号噪声比,使得蛋白质结构的详细建模,并揭示了新的见解.

更多相关视频

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.1K
Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

6.4K

相关实验视频

Last Updated: Jun 13, 2025

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

6.6K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.1K
Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

6.4K

科学领域:

  • * 相对显微镜和结构生物学.
  • *先进的电子结晶学技术.

背景情况:

  • * 在宏分子晶体学中获得高分辨率数据受到衰减的衍射强度和辐射损伤的限制.
  • * 直接电子探测器和电子计数能够在低流量下收集MicroED数据,但不弹性散射仍然是一个重要的噪声源.
  • *来自不弹性散射的噪声阻碍了对微弱,高分辨率反射的准确测量.

研究的目的:

  • * 调查能量过对MicroED数据质量和分辨率的影响.
  • * 评估能量过和电子计数用于宏分子结构确定的综合益处.
  • * 探索从扩散散散现象中获得的新型结构信息.

主要方法:

  • * 使用直接电子探测器进行电子计数,用于MicroED数据采集.
  • * 采用能量过器去除不弹性散射的电子.
  • *从蛋白酶K晶体收集和处理了MicroED数据.

主要成果:

  • * 能量过通过减少不弹性散射引起的背景噪声,显著改善了信号噪声比.
  • * 实现亚原子分辨率的MicroED数据,使得精确的结构建模和微细细节的可视化.
  • * 观察并描述了以前被噪声所掩盖的扩散散射现象.

结论:

  • *在MicroED中将能量过与电子计数相结合,可以提高数据的准确性和分辨率.
  • *这种方法有助于精确地细化蛋白质结构,并更深入地了解蛋白质的功能.
  • *通过降低噪声而显示的分散散射,为额外的结构洞察提供了潜在的潜力.