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Updated: Jun 13, 2025

Single Particle Cryo-Electron Microscopy: From Sample to Structure
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验证3D冷EM单颗粒重建的正确性和手性,使用Ewald的球体校正

Raquel Bromberg1,2, Yirui Guo1,2, Dominika Borek2,3

  • 1Ligo Analytics, 2707 Chunk Ct., Dallas, TX, 75206, United States.

bioRxiv : the preprint server for biology
|September 11, 2024
PubMed
概括

这项研究引入了一种新方法来分离电子显微镜中的散射贡献,提高3D重建的准确性. 该方法自动确定样本的手性,并验证重建质量.

关键词:
埃瓦尔德的球体纠正具有复杂值的图像.低温电子显微镜 单颗粒重建确定使用手的决定.验证验证的时间

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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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