在支气管支气管支气管洗液样本中对病原体诊断的甲基基因组和目标下一代测序进行比较分析
Weijie Sun1, Lin Zheng1, Le Kang2
1Clinical Laboratory, The First Affiliated Hospital of Ningbo University, Ningbo, China.
Frontiers in cellular and infection microbiology
|September 11, 2024
概括
甲基因组下一代测序 (mNGS) 和向下一代测序 (tNGS) 在检测肺部感染中的细菌和真菌方面表现出类似的有效性. 有针对性的NGS可能为DNA病毒检测提供优势.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 新一代测序 (NGS) 试验,包括超基因组 (mNGS) 和向性 (tNGS) 试验,越来越多地用于识别肺部感染的病原体.
- 有限的研究系统地比较了mNGS和多重PCR基tNGS在支气管支气管洗液 (BALF) 中的疗效.
研究的目的:
- 系统地评估和比较mNGS和基于多重PCR的tNGS在BALF标本中的病原体检测的诊断性能.
- 评估mNGS和tNGS在识别各种微生物方面的一致性和差异.
主要方法:
- 从怀疑感染性疾病的患者收集了85个BALF标本.
- 在所有收集的样本上执行并行mNGS和tNGS工作流.
- 使用PCR对临床关键病原体的差异结果得到证实.
主要成果:
- 对于mNGS (95.18%) 和tNGS (92.77%),微生物检测率都很高,没有显著差异.
- mNGS检测到55种微生物,而tNGS检测到49种病原体;两种方法之间观察到86.75%的一致性.
- 在特定的人类疹病毒的检测率中发现了显著的差异,tNGS显示出更高的灵敏度.
结论:
- mNGS和基于多重PCR的tNGS在BALF中检测细菌和真菌方面表现相似.
- 针对性NGS在检测DNA病毒方面可能优于mNGS.
- 对于临床关键的病原体,在NGS测定和PCR测定之间没有发现显著差异.
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