基于快速,高密度光谱扫描的便携式超光谱成像设置,用于in situ新鲜组织活检的定量生物化学映射
Luca Giannoni1,2, Marta Marradi1,2, Kevin Scibilia3
1University of Florence, Department of Physics and Astronomy, Florence, Italy.
Journal of biomedical optics
|September 11, 2024
概括
这项研究介绍了HyperProbe1,一种超光谱成像系统,用于快速地在现场对新鲜的外科活检进行生物化学分析. 它可以更快,非破坏性的组织查,改善质瘤的诊断和分类.
科学领域:
- 生物医学光学 生物医学光学
- 医疗成像医学成像
- 癌症的诊断 癌症的诊断
背景情况:
- 目前对手术活检 (例如质瘤) 的组织病理学分析是耗时的,延迟了临床决策.
- 现有的方法需要样本固定和染色,这可能会损害组织完整性并延长分析时间.
研究的目的:
- 开发和描述HyperProbe1,一个便携式高光谱成像系统,用于快速地在现场对新鲜外科组织进行生化分析.
- 为了证明系统对非破坏性,实时生化绘图和潜在瘤分类的能力.
主要方法:
- 使用光谱扫描超光谱成像 (HSI) 设置,配备超连续激光照明和声光调节过器.
- 获得的新鲜外科活检 (结质瘤) 的3D超立方体,具有高空间分辨率和光谱信息 (510900 nm).
- 采用快速光谱分离用于对血红蛋白和细胞染色体-c-氧化酶 (CCO) 等生物标志物的定量映射.
主要成果:
- 成功地将HyperProbe1应用于11个新鲜的质瘤活检,生成定量生物化学图.
- 确定了包括氧血球,脱氧血球和CCO.CO在内的关键生物标志物.
- 初步分析表明,基于脂质含量和CCO度,有可能区分质瘤等级.
结论:
- HyperProbe1提供了一个概念验证,用于手术活检的快速,定量,生物化学映射.
- 这种非破坏性的现场查方法可以显著简化手术后的组织病理学实践.
- 这项技术有可能加速像质瘤这样的大脑瘤的诊断工作流程.
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