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甲基转移酶的并行进化导致了Tabernaemontana elegans和Catharanthus roseus中的vobasine生物合成

Maisha Farzana¹, Matthew Bailey Richardson¹, Daniel André Ramey Deschênes¹

¹Department of Chemistry, University of New Brunswick, Fredericton, NB, Canada.

Frontiers in plant science

|September 11, 2024

概括

研究人员在树中发现了一种新的酶 - - 周边Nβ-甲基转移酶 (TePeNMT). 这一发现表明,在植物中,参与单类醇化物N-甲基化过程的酶的并行进化.

科学领域:

生物化学生物化学
植物科学植物科学
酶学是一种酶学.

背景情况:

单类醇类化合物 (MIAs) 是一种复杂的自然产品类别,具有重要的制药应用.
N-甲基化是MIA的一个关键修饰,影响其生物活性.
已知的MIA N-甲基转移酶 (NMTs) 从祖先的γ-托科菲罗尔C-甲基转移酶 (γTMTs) 进化,这是在Apocynaceae家族中观察到的现象.

研究的目的:

来自Tabernaemontana elegans的一种新型NMT的识别和描述.
为了研究这种新酶的进化起源和酶性质.
为了比较新发现的酶与以前描述的NMT.

主要方法:

遗传学分析以确定进化关系.
酶动力学实验以评估催化活性.
酶同质模型用于研究活性部位结构.

主要成果:

在Tabernaemontana elegans.中发现了一种新的 γTMT 类酶 - - 周边Nβ-甲基转移酶 (TePeNMT).
遗传学分析显示,TePeNMT及其同类构成一个独特的类.
酶动力学和同质模型显示了来自Catharanthus roseus的TePeNMT和CrPeNMT之间的显著差异.

结论:

这些发现表明,祖先的 γTMTs 在周围N-甲基化多样化中的并行进化.
这项研究突出了NMT在Apocynaceae家族中独特的进化途径.
TePeNMT的表征提供了对MIA修饰背后的生化机制的见解.

关键词:

酒吧蒙大拿优雅的人甲基转移酶的使用方法单烯酸体的烯酸体是单烯酸体的烯酸体.自然产品的生物合成.平行进化的平行演化.这就是vobasine.

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