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聚氨基素B辅助剂对抗聚氨基素B和耐卡巴胺的格拉姆阴性细菌

João Paulo Salvaterra Pasquini¹, Paula Assis Queiroz¹, Pedro Henrique Rodrigues do Amaral²

¹Postgraduate Program in Bioscience & Physiopathology, State University of Maringa, Maringa, Parana, Brazil.

Future microbiology

|September 11, 2024

概括

新的丁二酸衍生物 (DNH) 显示出作为辅助剂的承诺,以恢复对抗抗卡巴耐药格兰氏阴性细菌 (CR-GNB) 的Polymyxin B (PMB) 疗效. 这些化合物显著增强PMB的活性,为抗难治愈的感染提供了潜在的策略.

科学领域:

微生物学微生物学
药理学药理学是指药理学的学科.
传染性疾病传染性疾病

背景情况:

聚米辛B (PMB) 是对抗卡巴耐药格兰阴性细菌 (CR-GNB) 感染的关键抗生素.
对PMB的新兴耐药性需要新的治疗策略,包括抗生素辅助剂.

研究的目的:

评估3,5-丁酸衍生物 (DNH) 和异化-N-酸 (INZ) 作为辅助剂,以增强PMB对CR-GNB的疗效.
研究DNH衍生物恢复PMB活动的机制.

主要方法:

最低抑制度 (MIC) 和最小杀菌度 (MBC) 的测定.
药物组合测定使用Enterobacterales和Acinetobacter baumannii的临床分离物.
流细胞计和扫描电子显微镜 (SEM) 来评估细胞效应.

主要成果:

DNH01,DNH11和DNH20在80%的测试CR-GNB分离物中恢复了PMB活性 (MIC ≤2μg/ml).
DNH衍生物使PMB能够在256倍低的度下有效.
流细胞计和SEM证实了DNH衍生物增强的PMB活性.

结论:

DNH衍生物是有希望的辅助剂,可以增强PMB对抗PMB抗性CR-GNB的有效性.
这些化合物提供了一种潜在的治疗途径,可以对抗具有挑战性的阴性细菌感染.

关键词:

3,5-丁二enzoylhydrazonic衍生品的使用情况.细菌的耐药性细菌的耐药性它们是肠杆菌.异化 - 乙烯基化的衍生物异化.聚氨酸是多种类型的.

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