纳米材料封装的dsRNA向基通路――一种潜在的有效和环保的策略,用于对抗棉花,Aphis gossypii (Hemiptera: Aphididae)
Zi-Han Wei1, Peng Zhao1, Xin-Yuan Ning1
1Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Journal of agricultural and food chemistry
|September 11, 2024
概括
这项研究引入了一种新的SPc/双链RNA (dsRNA) /植物农药策略,用于控制棉花,Aphis gossypii. 这种环保的方法显著增加了害虫死亡率,并减少了化学杀虫剂的使用.
科学领域:
- 农业昆虫学 农业昆虫学
- 虫害管理 虫害管理 虫害管理
- 纳米技术 纳米技术
背景情况:
- 像棉花花 (Aphis gossypii) 这样的策略性害虫表现出快速增长和抗农药性,对作物生产构成重大挑战.
- 有效控制Aphis gossypii对于保护黄瓜和棉花产量至关重要.
- 现有的控制方法经常与害虫的复苏和耐药性的发展作斗争.
研究的目的:
- 开发和评估Aphis gossypii的增强害虫控制策略,使用SPc纳米载体,双链RNA (dsRNA) 和植物农药的组合.
- 为了研究植物农药对Aphis gossypii中丁通路基因的影响.
- 评估SPc/dsRNA/植物复合物在实验室和温室条件下增加害虫死亡率的有效性.
主要方法:
- 用植物性农药 (阿扎迪拉克,马林,欧卡利普托尔) 对Aphis gossypii进行治疗,以观察基因表达 (AgCHS2,AgHK2) 基因表达 (AgCHS2,AgHK2) 路径的变化.
- 制定一个SPc纳米载体系统,以提高dsRNA的稳定性,透性和干扰效率.
- 在受控环境中应用SPc/dsRNA/植物复合物来控制Aphis gossypii.
主要成果:
- 植物农药治疗改变了Aphis gossypii中基因通路基因AgCHS2和AgHK2的表达.
- SPc纳米载体显著改善了dsRNA的性能特征.
- 在实验室和温室环境中,SPc/dsRNA/植物复合体显示Aphis gossypii死亡率明显增加.
结论:
- 开发的SPc/dsRNA/植物战略提供了一种有效和环保的方法来控制Aphis gossypii.
- 这种方法提高了害虫死亡率,同时减少了对传统化学农药的依赖.
- 该策略的重点是保存的丁通路基因,这表明该策略可能适用于管理其他r-strategy害虫.
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