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在来自新热带蝙蝠的metatranscriptomic数据中发现和生物证实了高度分歧的塔卡里比病毒

Carlo Fischer¹, Murilo Henrique Anzolini Cassiano¹, William R Thomas²

¹Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt Universität zu Berlin, Institute of Virology, Berlin, Germany.

|September 11, 2024

概括

在多米尼加共和国的蝙蝠中发现了一种高度分歧的塔卡里比病毒 (TCRV) 菌株,使用了公共测序数据. 这一发现证实了TCRVV的存在.

科学领域:

病毒学病毒学
基因组学就是基因组学.
蝙蝠传播的病毒

背景情况:

塔卡里贝病毒 (TCRV) 是一种新世界舞台病毒 (NWA),首次在1956年被分离出来,但从那以后很少被检测到.
由于实验性感染和有限的基因组数据,TCRV与蝙蝠的关联受到质疑.
现有的TCRV基因组数据很少,在加勒比蝙蝠和美国虫中发现了近乎相同的菌株,相隔数十年.

研究的目的:

探索蝙蝠携带的NWAs的遗传多样性和地理分布.
为了识别新的TCRV菌株并确认它们与蝙蝠宿主之间的关联.
为了研究不同的TCRV菌株的致病性.

主要方法:

使用Serratus搜索570万次公开测序运行NWA读数.
从蝙蝠心脏和眼睛组织的metatranscriptomic数据中恢复并组装了一个完整的TCRV基因组.
通过桑格测序来自原始蝙蝠肝样本的PCR amplicon确认病毒存在和基因组完整性.

主要成果:

在来自多米尼加共和国的牙买加果蝙蝠中发现了一种高度分歧的TCRV菌株 (DOM2014).
DOM2014菌株与其他TCRV菌株共享83.3%-86.0%的基因组特征和91.8%-93.7%的氨基酸特征.
DOM2014 具有一个保存的 GPPT 核蛋白动机,对于干扰素对抗至关重要.
感染蝙蝠的肝脏组织中病毒载量最高.

结论:

甲转录组分析成功识别了一种不同的TCRV菌株,证实了它与新热带蝙蝠 (Artibeus*属) 的关联.
由于TCRV的遗传多样性和地理分布,因此需要对菌株特异性致病性进行进一步的研究,特别是对新型DOM2014菌株进行进一步的研究.
通过原始样本测试验证基因组发现,加强了转录基因组学在病毒学研究中的实用性.

关键词:

塔卡里贝贝 (Tacaribe) 是一个名字.竞技场病毒病毒蝙蝠蝙蝠蝙蝠蝙蝠是一个基因组学就是基因组学.病毒学病毒学病毒的演变病毒的演变

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