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击败PAIP1通过调节环林E2mRNA稳定性来抑制乳腺癌细胞的增殖

Wenqing Yang^1,2, Qingkun Wang^1,2, Qi Li³

¹Key Laboratory of Pathobiology, State Ethnic Affairs Commission, Yanbian University, Yanji, China.

Molecular carcinogenesis

|September 11, 2024

概括

聚氨基酸结合蛋白相互作用蛋白1 (PAIP1) 驱动乳腺癌的进展. 沉默PAIP1通过降低循环E2mRNA稳定性来抑制癌细胞的生长,增殖和存活,这表明PAIP1是治疗点.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
生物化学生物化学

背景情况:

聚氨基酸结合蛋白相互作用蛋白1 (PAIP1) 涉及翻译启动和癌症进展.
在包括乳腺癌在内的各种癌症中观察到PAIP1过度表达,与预后不佳相关.
在乳腺癌中PAIP1的确切作用和机制尚不完全理解.

研究的目的:

阐明PAIP1在乳腺癌进展中的生物功能和分子机制.
在体外和体外研究PAIP1对乳腺癌细胞行为的影响.
确定受PAIP影响的下游目标和监管途径1.

主要方法:

构建PAIP1抑制的乳腺癌细胞系.
细胞增殖,细胞周期分布和细胞亡的评估.
用于目标预测和机械探索的RNA测序 (RNA-seq).
在体内验证的异种移植小鼠模型.

主要成果:

降低PAIP1显著抑制了乳腺癌细胞的增殖,诱导细胞循环停止,并触发了亡.
在体内研究表明,PAIP1倒置在异种移植小鼠模型中抑制瘤生长.
RNA-seq确定了CCNE2mRNA作为下游目标,PAIP1敲击通过调节mRNA稳定性来降低环林E2表达.
在人类乳腺癌组织中观察到PAIP1和环素E2表达之间的正相关性.

结论:

PAIP1通过调节环林E2表达和mRNA稳定性,在促进乳腺癌进展方面发挥着至关重要的作用.
抑制PAIP1为乳腺癌治疗提供了一个潜在的治疗策略.
向PAIP1可能是对抗乳腺癌的新方法.

关键词:

这就是PAIP1的原因.环 E2 环 E2 环 E2 是一种基因-微型阵列在mRNA的稳定性上.它们的扩散和扩散.

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