在年轻和老年骨肌肉中,对葡萄糖皮质激素敏感的转录组的收缩调节
Grant R Laskin1, David S Waddell2, Cynthia Vied3
1Department of Health, Nutrition and Food Sciences, Florida State University, Tallahassee, Florida, United States.
American journal of physiology. Endocrinology and metabolism
|September 11, 2024
概括
运动有助于抵制葡萄糖皮质类药物引起的肌肉衰弱,特别是在老年人中. 肌肉收缩反对德克萨米他的基因表达变化,特别是在老年肌肉中,PPARG发挥着关键作用.
科学领域:
- 骨肌肉生理学 骨肌肉生理学
- 分子生物学分子生物学
- 衰老研究研究 衰老研究
背景情况:
- 葡萄糖皮质醇的升高会诱导肌肉病,其特征是缩,虚弱和代谢功能障碍.
- 老年肌肉更容易受到葡萄糖皮质醇诱导的肌肉病变的影响.
- 抵抗运动可以减轻年轻肌肉中的葡萄糖皮质菌病,但对老年肌肉的影响尚不清楚.
研究的目的:
- 调查肌肉收缩的能力,以抵消葡萄糖皮质类药物诱导的转录组变化在年轻和老年骨肌肉.
- 确定参与这种保护作用的分子机制,包括特定的转录因子.
主要方法:
- 年轻和年老的小鼠接受了德甲 (DEX) 或盐水.
- 在注射后,前肌肉经历了高强度的收缩.
- RNA测序分析了葡萄糖皮质体和收缩敏感基因.
- 在分析和基因操纵 (PPARG) 探索了分子途径.
主要成果:
- 肌肉收缩对抗了甲诱导的基因表达变化,在年轻和老年肌肉中,在老年肌肉中更有效.
- 与代谢 (年轻) 和肌肉大小/炎症 (老年) 相关的收缩相反的基因程序.
- 氧酶增殖器激活受体玛 (PPARG) 被确定为调解衰老肌肉中收缩诱导的对抗的关键因素.
结论:
- 肌肉收缩可以显著反对葡萄糖皮质醇对老化骨肌肉转录组的有害影响.
- PPARG激活是一种潜在的机制,通过该机制,运动可以抵消葡萄糖皮质醇诱导的老化肌肉的变化.
- 这些发现增强了对运动如何调节老年肌肉在压力下基因表达的理解.
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