棒吸附微提取和液体色谱-二极管阵列检测合成大麻素在口腔液体中的检测
Samir M Ahmad1, Nuno R Neng2, Cláudio R Queirós3
1Centro de Química Estrutural, Institute of Molecular Sciences, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisboa, Portugal.
Analytical and bioanalytical chemistry
|September 11, 2024
概括
使用棒吸附微提取和HPLC-DAD的新分析方法被开发用于检测口服液中的八种合成大麻素 (SC). 这种方法提供了一种可靠和有效的方法来监测生物样本中的这些物质.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
- 药理学 药理学是指药理学的学科.
背景情况:
- 合成大麻素 (SCs) 构成了严重的公共卫生问题.
- 需要先进的分析技术来检测生物矩阵中的SCs.
- 口服液分析为药物监测提供了一个非侵入性的替代方案.
研究的目的:
- 开发和验证一种新的分析方法来选口腔液样本中的八种特定的SCs.
- 为了优化棒吸附微提取,然后通过微液溶解 (BAμE-μLD) 技术与HPLC-DAD相结合.
- 评估用于常规SC监测的开发方法的性能.
主要方法:
- 开发了一种棒吸附微提取 (BAμE) 与微液溶解 (μLD) 结合的方法.
- BAμE-μLD与高性能液体染色学与二极管阵列检测 (HPLC-DAD) 的合.
- 提取参数的优化和口服液体矩阵的分析方法的验证.
主要成果:
- 该方法在20.02000.0μg L-1.1的动态范围中显示出良好的线性 (r2 > 0.99).
- 在八个目标SC中实现了低检测极限 (2.05.0μg L-1) 和高回收率 (87.9100.5%).
- 显示了可接受的日内和日间准确性 (偏差 ≤ ±14.7%) 和精度 (RSD ≤ 14.9%).
结论:
- 经过验证的BAμE-μLD/HPLC-DAD方法在口服液中查目标SCs方面非常有效.
- 该方法显示出出色的性能特征,使其适合常规监测.
- 这种新的方法为法医毒理学和公共卫生监测SC使用提供了有价值的工具.
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