在低毒条件下,在HIF1A中介细胞干细胞中被击败后,Histon H3K9甲基化特征In Vitro
P I Bobyleva1, E A Tyrina2, M V Lobanova2
1Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia. blastoblast@gmail.com.
Bulletin of experimental biology and medicine
|September 11, 2024
概括
低氧刺激介质细胞中体细胞中的组素H3K9甲基化,其中HIF1A调节关键基因. 这种依赖HIF-1的机制在正常的氧气条件下也可运行,影响H3K9甲基化.
科学领域:
- 细胞生物学 细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 介酶体 stromal 细胞 (MSCs) 对于组织修复和再生至关重要.
- 像H3K9甲基化这样的质子修饰,在调节基因表达方面发挥着至关重要的作用.
- 缺氧是影响细胞行为和表观遗传调节的关键环境因素.
研究的目的:
- 为了研究缺氧对人类带MSCs中希斯H3K9甲基化的影响.
- 阐明缺氧诱导因子1-alpha (HIF1A) 在低氧和正常条件下调节参与H3K9甲基化中的基因的作用.
主要方法:
- 对于HIF1A的RNA干扰 (RNAi) 进行敲击.
- 对基因转录活性进行定量分析 (KDM3A,KDM4A,EHMT2).
- 对抗原表达和H3K9甲基化水平的流细胞计.
主要成果:
- 缺氧显著刺激H3K9甲基化和MSCs中相关基因的表达.
- HIF1A调节KDM4A和EHMT2的表达,从而调节蛋白质水平.
- KDM3A,KDM4A,EHMT2/G9a和H3K9甲基化的HIF1A依赖调节在正常和缺氧的情况下都发生.
结论:
- 缺氧诱导MSC的表观遗传变化,特别是通过HIF1A增强H3K9甲基化.
- HIF1A是MSCs表观遗传机制的关键调节者,在不同氧含量下影响H3K9甲基化.
- 研究结果表明,HIF1A在MSC内的表观遗传控制中具有保留的调节作用.
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