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一种基于八甲基结合的赫托里特的QuEChERS方法,用于确定雅克吉的十种真菌毒素

Jun Wang¹, Hai-Yan Yang¹, Jia-Jia Fan¹

¹Zhang Zhongjing School of Chinese Medicine, Nanyang Institute of Technology, Nanyang, 473004, China.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

|September 11, 2024

概括

一种新的八甲基结合的赫托里特材料有效地清理高脂肪的雅克油样本,以检测真菌毒素. 这种方法使用QuEChERS-HPLC-MS/MS精确量化了十种真菌毒素,满足了食品安全要求.

科学领域:

食品化学食品化学
分析化学分析化学
材料科学材料科学材料科学

背景情况:

雅克油等食品中的菌毒素污染对健康构成重大风险.
在复杂的高脂肪矩阵中分析真菌毒素,例如雅克吉,由于干扰物质,这给分析带来了挑战.

研究的目的:

为高脂肪食物矩阵合成一种新的清洁材料.
开发和验证一种敏感的分析方法,用于检测雅克格里的多种真菌毒素.

主要方法:

通过多步骤化学反应合成与八甲基结合的赫克托 (Hectorite@NHCO(CH2) 17CH3).
开发一种经过修改的QuEChERS (快速,轻松,廉价,有效,坚固,安全) 提取方法,与高性能液体染色学-并列质谱法 (HPLC-MS/MS) 相结合.
在分析之前,利用Hectorite@NHCO(CH2)17CH3从雅克格里提取物中选择性去除干扰物质.

主要成果:

合成的Hectorite@NHCO(CH2)17CH3证明了高脂肪样品的有效清洁能力.
在QuEChERS-HPLC-MS/MS方法中,在1.0-500μg/kg范围内显示出优异的线性 (R2 ≥0.9991).
达到了十种真菌毒素的低检测值 (0.1018.62μg/kg) 和定量值 (0.3262.07μg/kg).
恢复率在72.2%至113.9%之间,相对标准偏差 (RSD) 在3.2%至17.5%之间,表明高准确度和精度.

结论:

使用Hectorite@NHCO(CH2)17CH3开发的方法非常准确和精确,可以检测雅克油中的十种真菌毒素.
这种分析方法有效地解决了高脂肪食物矩阵中分析真菌毒素的挑战.
该方法适用于例行监测,并确保雅克吉产品的安全.

关键词:

清理材料清理材料清理材料黑克托里特 (hectorite) 是一种矿.菌类毒素 (Mycotoxins) 是一种毒素.奎瑟人奎瑟人雅克格希 (Yak ghee) 是一种油脂.

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