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Updated: Jun 13, 2025

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

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蛋白质两极化干电荷对相对蛋白质干结合 afinities 的影响

Suliman Adam1, Itamar Kass1, Dana Krepel-Zussman1

  • 1InterX LTD (a Subsidiary of NeoTX Therapeutics Ltd), 2 Pekeris Street, Rehovot 7670202, Israel.

Journal of chemical theory and computation
|September 11, 2024
PubMed
概括

预测分子结合能量对于药物设计至关重要. 一种新的方法,蛋白质诱导极化 (PIP) 收费,通过计算中考虑蛋白质效应来提高准确性.

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In...
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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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