一个基本的螺旋环螺旋转录因子VvbHLH036调节了葡萄藤的耐寒性
Yujun Hou1,2, Darren Chern Jan Wong3,4, Xiaoming Sun1
1CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture/Center of Economic Botany, Core Botanical Gardens/Sino-Africa Joint Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
Plant physiology
|September 11, 2024
概括
葡萄树对寒冷的耐受性由VvbHLH036增强,这种转录因子通过VvThrC1.6调节通过VvThrC1.6调节氨酸生物合成的转录因子. 这一途径改善了冷应激反应和葡萄中的氨基酸积累.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 寒冷压力显著限制了葡萄藤 (Vitis vinifera) 的生长和生产力.
- 了解耐寒性分子机制对于作物改善至关重要.
研究的目的:
- 识别和描述参与寒冷应激反应的葡萄葡萄基因.
- 阐明VvbHLH036的调节作用及其下游冷耐受性目标.
主要方法:
- 基因鉴定和鉴定 (VvbHLH036).
- 葡萄葡萄中的基因过度表达和CRISPR/Cas9介导的淘汰赛 (KO).
- 转录组分析和促进体结合试验.
- 测量氨酸含量和活性氧物种 (ROS).
主要成果:
- VvbHLH036积极调节葡萄树根的寒冷耐受性.
- VvbHLH036直接激活了三氨酸合成酶 (VvThrC1) 的表达.
- 寒冷压力诱导VvThrC1和氨酸的积累,由VvbHLH036调节;外源性氨酸增强了寒冷耐受性.
结论:
- VvbHLH036和VvThrC1是葡萄中氨酸生物合成和冷应激反应的关键调节者.
- 这一途径通过调节氨基酸水平并减少氧化损伤来提高耐寒性.
- 这些发现为开发耐寒葡萄品种提供了潜在的目标.
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