烯基异构酶FKBP11是一种分泌的转位子辅助因子
Amanda DiGuilio1, Ben Cheng1, Frank Zhong2
1Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637.
FKBP11,是内质网膜 (ER) 中的一种普罗利尔异构酶,与核糖体 - 转环子复合体结合. 这种辅助因子有助于分泌和膜蛋白的合成和折叠.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 蛋白质折叠 蛋白质的折叠
背景情况:
- 细胞合成成千上万的分泌和膜蛋白,这些蛋白质往往通过转换转移到内质网膜 (ER).
- 在ER中蛋白质折叠涉及分子伴侣和酶,而proline-isomeric过渡是潜在的速度限制步骤.
- 六种人类ER prolyl异相酶的特定功能和基质尚不清楚.
研究的目的:
- 为了研究ER中prolyl异构酶FKBP11的功能和结合特性.
- 确定FKBP11在分泌和膜蛋白的合成和稳定性中的作用.
主要方法:
- 通过其跨膜域和C端区域识别FKBP11与核糖体-转基因复合体 (RTC) 的结合.
- 使用高通量mRNA测序来分析核糖体参与.
- 在缺乏FKBP11的细胞中进行功能分析,以评估蛋白质的稳定性.
主要成果:
- FKBP11选择性地与RTCs结合,合成具有广泛转位段的分泌和膜蛋白.
- FKBP11的耗尽导致了 EpCAM 和 PTTG1IP 等特定蛋白质的稳定性降低.
- FKBP11作为一个转位子辅助因子,影响ER的蛋白质合成.
结论:
- FKBP11是一种特定于甲动物的基异构酶,作为转位子辅助因子起作用.
- 它在合成和折叠各种可溶性分泌蛋白和跨膜蛋白中发挥着至关重要的作用.
- 这一发现澄清了FKBP11在ER蛋白加工中的作用.
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