循环RNA RRM2通过向miR-142-5p来增加NRG1表达来缓解与代谢功能障碍相关的脂肪性肝病
Long-Fei Wu1,2,3, Zhi-Jiang Zhou1, Yu-Heng Zeng1
1Department of Preventive Medicine, Shantou University Medical College, Shantou, People's Republic of China.
American journal of physiology. Gastrointestinal and liver physiology
|September 11, 2024
概括
循环RNA核糖核酸减少酶子单元M2 (circRRM2) 在与代谢功能障碍相关的脂肪性肝病 (MASLD) 中被下调. 过度表达circRRM2通过海绵化microRNA-142-5p和调节神经蛋白1来减少甘油三积累,从而减轻MASLD的进展.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 与代谢功能障碍相关的脂肪性肝病 (MASLD) 是一个日益严重的全球健康问题.
- 循环RNAs (circRNAs) 在肝病中起着调节作用,但它们在MASLD病原发生中的具体参与尚未完全理解.
研究的目的:
- 调查循环RNA核糖核酸减少酶子单元M2 (circRRM2) 在MASLD进展中的作用.
- 阐明在MASLD中涉及circRRM2,microRNA-142-5p (miR-142-5p) 和神经调节素1 (NRG1) 的分子机制.
主要方法:
- 建立了体外 (用自由脂肪酸处理的肝细胞) 和体内 (高脂肪饮食引起的) MASLD 模型.
- 使用RT-qPCR对circRRM2,miR-142-5p和NRG1的量化表达水平.
- 经过验证的分子相互作用使用双露西法酶记者测定和体外/体内活体功能实验.
主要成果:
- 在MASLD模型中,circRRM2和NRG1的表达减少,而miR-142-5p在MASLD模型中升高.
- 在MASLD小鼠中,circRRM2的过度表达减少了脂质生成和甘油三积累.
- circRRM2作为miR-142-5p的海绵,NRG1是miR-142-5p的目标,共同调节MASLD的进展.
结论:
- circRRM2通过调节miR-142-5p/NRG1轴在MASLD中发挥保护作用.
- circRRM2可能成为MASLD治疗的潜在治疗点.
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