埃舍里希亚大肠杆菌的新陈代谢工程用于基基氨基生产
Hulin Yang1, Shizhuo Wang1, Meiyi Zhao1
1Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China.
Journal of biotechnology
|September 11, 2024
概括
通过工程设计的大肠杆菌产生补充剂的甲基囊蛋白 (SeMCys). 代谢工程策略,包括基因删除和途径增强,显著提高了SeMCys对潜在治疗应用的产量.
科学领域:
- 微生物生物技术 微生物生物技术
- 代谢工程是代谢工程.
- 的新陈代谢 的新陈代谢
背景情况:
- (Se) 是人类健康的重要微量元素.
- 甲基氨基 (SeMCys) 作为一种具有抗癌和增强认知功能的Se补充剂,显示出有前途.
- 微生物生产为SeMCys合成提供了一个可扩展的途径.
研究的目的:
- 为了设计大肠杆菌以实现SeMCys.的高效微生物生产.
- 通过代谢工程策略优化SeMCys的产量.
- 研究工程化大肠杆菌中的Se代谢.
主要方法:
- 大肠杆菌的基因工程通过将SeMCys合成分为三个模块 (氨基氨酸合成,甲基供体合成,SMT).
- 优化基因表达使用具有不同副本数量的等离子体.
- 通过基因淘汰 (csdA, sufS) 修改SeCys降解途径.
- 增强血清素合成途径和食批量培养与胺补充剂.
主要成果:
- 增加SeCys合成模块的副本数量提高了SeMCys的生产.
- 删除csdA或sufS,特别是两者,显著改善了SeMCys的产量.
- 添加氨酸和增强其合成途径增加了12.5%的SeMCys产量.
- 食批量培养实现了SeMCys产量为3.715 mg/L的产量.
结论:
- 大肠杆菌的代谢工程是SeMCys生产的可行策略.
- 特定的基因修饰和培养条件对于最大化SeMCys产量至关重要.
- 这项研究提供了对微生物Se代谢和SeMCys生物合成的基础见解.
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