对含有氧利平的脂的LC-HRMS分析
Laura Carpanedo1, Katharina M Rund1, Luca M Wende1
1Faculty of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany.
Analytica chimica acta
|September 11, 2024
概括
这项研究引入了一种新的液体染色学高分辨率质谱法 (LC-HRMS) 方法,用于直接分析脂 (PL) 中的氧利平. 该方法揭示了氧化位置如何影响氧利平在人体细胞中的不同PL类的纳入.
科学领域:
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 氧利平,包括氧和环氧多不和脂肪酸,是重要的脂质介质.
- 这些氧利平存在于非化和化两种形式,主要存在于脂 (PL) 中.
- 目前的间接量化方法丢失了关于特定脂类结合氧利平的信息.
研究的目的:
- 开发一种非向液体染色学高分辨率质谱法 (LC-HRMS) 方法,用于直接分析与PL结合的氧利平.
- 为了识别和描述含有氧利平的单个PL物种.
- 研究人类细胞中特定氧利平的纳入不同PL类的过程.
主要方法:
- 利用优化逆相LC分离来解决异和异的PL物种.
- 采用高分辨率质谱测量来识别基于保留时间,前体和产品离子的PL物种.
- 通过α-裂变产物离子来表征结合的氧利平,并使用中性损失的sn-2脂肪酸来确定PL异构体.
主要成果:
- 在7个脂类 (PI,PS,PC,PE,PC-P,PC-O,PE-P) 中检测到422个含氧利平因的个人PL物种在人体血清和细胞中.
- 成功确定了HEK293细胞中补充氧利平的PL类合并.
- 观察到的特定的纳入模式:20:4;15OH,20:4;14Ep和20:5;14Ep主要在PI中;20:4;8Ep和20:5;8Ep在PC和PE中;其他氧利平主要在PC中.
结论:
- 开发的LC-HRMS方法允许全面检测和半量化各种含氧利的PL物种.
- 证明氧化位置显著影响并指导氧利平在人体细胞中被纳入不同的PL类.
相关概念视频
Asymmetric Lipid Bilayer
7.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.2K
Membrane Lipids
23.0K
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
23.0K
Structure of Lipids
87.4K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
87.4K
What are Lipids?
7.5K
Lipids function as structural components of cellular membranes, in addition to acting as energy reservoirs and signaling molecules. They are thus crucial to all living organisms. The three biologically important classes of lipids are triglycerides, phospholipids, and steroids.
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and...
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and...
7.5K
Overview of Fatty Acid Metabolism
30.3K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
30.3K
Synthesis of Phosphatidylcholine in the ER Membrane
3.0K
The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...
The major components of all eukaryotic cell...
3.0K


