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从干细胞获得的人类植入后胚胎模型的组装

Carlos W Gantner¹, Bailey A T Weatherbee¹, Yuntao Wang¹

¹Mammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.

Nature protocols

|September 11, 2024

概括

研究人员从干细胞中制造出人类可诱导胚胎 (hiEmbryoids),以模拟人类早期发育. 这一突破使得研究胚胎和胚胎外组织相互作用在植入阶段.

科学领域:

发展生物学发展生物学
干细胞生物学干细胞生物学
人类胚胎发生

背景情况:

人类胚胎和胚胎外组织在植入过程中的相互作用尚不清楚.
在人类胚胎中研究这些早期发育阶段是具有挑战性的.
多能干细胞为研究人类早期发育提供了一个模型系统.

研究的目的:

开发一种可处理的体外模型,用于研究人类胚胎在植入时的形成.
调查特定转录因子在胚胎外血统规范中的作用.
从人类干细胞中创建自我组织的胚胎状结构.

主要方法:

在人类胚胎干细胞 (ES) 中激活特定的转录因子 (GATA6,SOX17,GATA3,TFAP2C),以诱导胚胎外类似的细胞命运.
诱导的胚胎外样细胞与野生类型的ES细胞共同培养,以形成自我组织的结构 (人类可诱导胚胎,hiEmbryoids).
对高胚胎发育的观察,包括表皮质极化,分化和胚胎外血统的规范.

主要成果:

人类ES细胞可以通过激活特定的转录因子转化为外胚性细胞.
这些细胞的共同培养产生了自我组织的人类可诱导胚胎 (hiEmbryoids).
hiEmbryoids回顾了早期人类发育的关键方面,包括表皮质分化和胚胎外组织形成.

结论:

人类诱导胚胎为研究植入前人类发展提供了一个新型模型.
这种模型允许对胚胎外胚胎相互作用进行可处理的实验.
该方案是高效的 (7-10天) 和可访问的研究人员,基本的干细胞专业知识.

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