来自泰国儿童的Streptococcus mutans的多部位序列类型化和表型性质,这些儿童有不同的病状态
Jinthana Lapirattanakul1, Ryota Nomura2,3, Rena Okawa2
1Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Rajthevi, Bangkok, 10400, Thailand. jinthana.lap@mahidol.ac.th.
BMC oral health
|September 11, 2024
概括
在儿童中Streptococcus mutans (S. mutans) 的基因定型没有将特定的克隆与状况联系起来. 虽然S. mutans的检测因的严重程度而有所不同,但其遗传构成和与酸有关的特征与牙没有明确的关联.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 牙健康 牙健康
背景情况:
- 突变性链球菌 (S. mutans) 是牙虫发展中的关键细菌.
- 突变菌表现出酸性和酸性特性,这对其化潜力至关重要.
- 之前的研究发现了S. mutans基因型和表型的变异.
研究的目的:
- 调查泰国儿童中S. mutans的基因型和表型特征.
- 为了将S. mutans的基因型和表型与不同的牙损伤状况相关联.
主要方法:
- 收集了395名3-4岁儿童的唾液样本,以确定S. mutans的存在和病状况.
- 选择了90名患有空,低或高空严重程度的儿童进行隔离采集.
- 使用多位元序列类型 (MLST) 进行基因型化270个S. mutans分离物,并评估生物膜形成,原结合和酸生产/耐受性.
主要成果:
- 在患有病变的儿童中,观察到更高的S. mutans检测率和计数.
- 来自高病严重程度 (HC) 组的S. 突变体显示生物膜形成减少,但对原蛋白的结合增加.
- MLST分析没有鉴定出S. mutans克隆特异性的空状态,也没有根据宿主空状态区分表型.
结论:
- 根据MLST的确定,S. mutans的克隆关系与宿主牙状况无关.
- S. mutans分离体的表型性质与特定的病严重程度或MLST定义的克隆不一致.
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