快速,简单和可重复的指纹方法用于纳米纤维素和酸基基凝支架中的内毒素表征
Jan Zuber1, Paula Lopes Cascabulho2,3,4, Sara Gemini Piperni5
1Department of Analytical Chemistry, TU Freiberg, Leipziger Street 29, 09599 Freiberg, Germany.
Biomacromolecules
|September 12, 2024
概括
纤维素水凝对伤口愈合有希望,但面临FDA内毒素限制. 需要新的灭菌方法来确保医疗应用的安全性和生物相容性.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 生物相容性测试 生物相容性测试
背景情况:
- 纳米纤维素和酸盐水凝正在探索伤口愈合.
- 关于内毒素水平的FDA规定限制了它们的临床使用.
- 有效的灭菌对于医疗级生物材料至关重要.
研究的目的:
- 评估灭菌方法对纳米纤维素水凝内毒素水平和细胞毒性的影响.
- 开发一种用于描述水凝中的内毒素的新方法.
- 评估纳米纤维素水凝对皮肤纤维细胞生长的生物相容性.
主要方法:
- 自动和水凝的紫外线灭菌.
- 电子喷雾电离 里埃转换离子循环子子共振质谱 (ESI FT-ICR MS) 用于内毒素指纹.
- 基于resazurin的测定用于评估人类皮肤纤维细胞活力和代谢活性.
主要成果:
- 纤维素纳米纤维 (CNF) 水凝在6欧盟mL-1显示略高的内毒素,但保持了细胞活力.
- 纤维素纳米晶 (CNC) 水凝降低了纤维细胞的代谢活动.
- 内毒素缺乏酸盐,但含有疏水的硫酸盐组.
结论:
- 对于医疗级纳米纤维素水凝来说,标准的灭菌方法是不够的.
- 由于内毒素的组成,CNC水凝具有潜在的细胞毒性.
- 新的高压杀菌技术是安全的医疗应用这些基于生物的水凝所需的.
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