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净化,从HK35的Pleurotus ostreatus中鉴定乳糖,并使用Box-Behnken设计优化刚果红色生物染色

Jantiya Isanapong¹, Kittikarn Suwannoi¹, Surangkana Lertlattanapong¹

¹Faculty of Applied Science, Department of Agro-Industrial, Food and Environmental Technology, King Mongkut's University of Technology North Bangkok, 1518 Pracharat 1, Wongsawang, Bangsue, Bangkok, 10800 Thailand.

|September 12, 2024

概括

研究人员从Pleurotus ostreatus真菌中净化了laccase,用于刚果红色的脱色. 优化的工艺实现了91.05%的效率,证明了其在去色剂应用中的潜力.

科学领域:

酶学是一种酶学.
生物技术是生物技术.
环境科学环境科学

背景情况:

乳糖酶对于降解回收性化合物至关重要.
白色腐烂真菌,如Pleurotus ostreatus,是已知的强效乳酸酶的来源.
刚果红色染料由于其持久性和毒性,给环境带来了挑战.

研究的目的:

为了净化和描述Pleurotus ostreatus HK35.35中的乳糖.
研究这种乳糖酶在脱色刚果红色染料中的应用.
使用统计设计优化去色化过程.

主要方法:

使用硫酸沉,透析,离子交换染色学和超过的酶净化.
酶的表征包括分子量,最佳温度和pH值的确定.
刚果红色脱色试验通过Box-Behnken设计进行优化,并使用ANOVA进行分析.

主要成果:

净化了一种具有15.26U/mg的特异活性和30.21%的回收的laccase.
该酶在60°C和pH值为4.0时表现出最佳活性,稳定性高达50°C.
优化的条件产生了91.05%的刚果红色脱色效率.

结论:

来自Pleurotus ostreatus HK35的净化乳液对刚果红色色变色是有效的.
该酶具有有利的特性,可用于染料废水处理中的工业应用.
统计优化显著提高了脱色效率,验证了模型的有效性.

关键词:

盒子Behnken设计设计刚果红色刚果红色刚果红色脱色的方法是脱色.拉卡斯 (Laccase) 是一个古老的城市.斑点鸟鱼 HK3535 的情况.净化净化净化净化

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