米尔蒂龙通过激活caspase 3来诱导结肠直肠癌中的GSDME依赖性烧
Guangwei Zheng1,2, Zhipeng Fang1,2, Zhenlv Lin1,2
1Department of Emergency Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.
Heliyon
|September 12, 2024
概括
米尔蒂龙通过触发气体皮质素E (GSDME) 依赖性热,一种编程细胞死亡途径,降低结肠直肠癌 (CRC) 细胞活力. 这表明米尔蒂龙是治疗CRC治疗的潜在治疗剂.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞死亡研究 细胞死亡研究
背景情况:
- 结肠直肠癌 (CRC) 是全球癌症相关死亡的主要原因.
- 炎症性编程细胞死亡的Pyroptosis是由GSDM家族调解的,在癌症治疗中显示出潜力.
- 了解CRC的新治疗机制至关重要.
研究的目的:
- 为了研究Miltirone对结肠直肠癌 (CRC) 细胞的影响.
- 为了阐明 pyroptosis,特别是 gasdermin E (GSDME) 在 Miltirone 抗癌活性中的作用.
- 探索涉及酶3的潜在分子机制.
主要方法:
- 用米尔蒂龙治疗的CRC细胞系 (SW620,HCT116) 进行细胞活力测定.
- 评估GSDME裂变和烧诱导的情况.
- GSDME抑制研究和caspase 3活性测定 (siRNA,特定抑制剂Z-DEVD-FMK).
主要成果:
- 米尔蒂龙显著降低了CRC细胞活力.
- 米尔蒂龙诱导了CRC细胞中的GSDME裂变和烧.
- 抑制GSDME或caspase 3减弱的米尔蒂龙诱导的热,证实了GSDME依赖的细胞死亡.
结论:
- 米尔蒂龙有效地诱导结直肠癌细胞中的GSDME依赖性热.
- 米尔蒂龙通过利用热致死来证明作为CRC的治疗剂的潜力.
- 这项研究为针对GSDME介导的热灭菌的新型CRC治疗提供了有希望的途径.
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