唾液皮质醇和皮质在长期储存后是稳定的
Marcus Imamovic1, Nils Bäcklund1, Staffan Lundstedt2
1Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden.
Scandinavian journal of clinical and laboratory investigation
|September 12, 2024
概括
唾液皮质醇和皮质素度在冷保存在-80°C时至少保持六年稳定. 这一发现支持冷唾液样本在压力和内分泌疾病研究中的长期可靠性.
科学领域:
- 内分泌学 在内分泌学.
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 冷的唾液样本对于研究压力和内分泌疾病至关重要.
- 精确测量唾液中的皮质醇和皮质素等皮质醇和皮质醇对研究至关重要.
研究的目的:
- 为了评估皮质醇和皮质素在唾液中的长期稳定性.
- 为了验证在-80°C保存六年的唾液样本的可靠性.
主要方法:
- 分析了153个冷的唾液分量,这些分量在-80°C下保存了6年.
- 使用液体染色学并联质谱仪进行精确量化.
- 重复测量与初始分析进行了比较.
主要成果:
- 对于皮质醇 (r=0.96) 和皮质素 (r=0.98) 的初始和重复测量之间观察到高度一致.
- 通过巴布洛克回归分析证实度偏差最小.
- 唾液皮质醇和皮质素水平在六年深储存后基本保持不变.
结论:
- 在-80°C下保存六年的唾液样本保持了皮质醇和皮质素度的完整性.
- 长期储存在-80°C是一个可靠的方法来保存唾液糖皮质激素用于研究.
- 这种稳定性支持在对压力和内分泌功能的纵向研究中使用历史的唾液样本.
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