IL-6,miRNA-146a,MALAT1遗传多态性与类风湿性关节炎风险之间的关联
Yasser Bm Ali1, Noura Ma Hasan1, Eman A El-Maadawy1
1Molecular Biology Department, Genetic Engineering & Biotechnology Research Institute (GEBRI), University of Sadat City, 32958, Egypt.
Personalized medicine
|September 12, 2024
概括
特定的IL-6,microRNA146a和MALAT1的遗传变异与埃及人的类风湿性关节炎 (RA) 风险有关. 在RA患者中这些基因的表达增加表明在疾病发展中发挥了作用.
科学领域:
- 遗传学 遗传学 是一个
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
背景情况:
- 类风湿性关节炎 (RA) 是一种慢性自身免疫性疾病.
- 遗传因素在RA易感性方面发挥着作用.
- 特定的基因多态可能会影响RA风险.
研究的目的:
- 在埃及人群中调查IL-6,微RNA146a和MALAT1和RA敏感性中单核酸多态 (SNPs) 之间的关联.
- 在RA患者中评估IL-6,microRNA146a和MALAT1的表达水平.
主要方法:
- 在101名RA患者和104名对照人群中,对IL-6 (-174G/C),microRNA146a (rs2910164C/G) 和MALAT1 (rs619586A/G) SNP进行基因定型.
- 使用酶相关免疫吸收试验 (ELISA) 分析IL-6的表达式,并使用对miR-146a和MALAT1.1的定量实时PCR (qRT-PCR) 进行定量实时PCR.
主要成果:
- 鉴定IL-6-174 GC基因型和C等位基因,miR-146a CG基因型和MALAT1 AA基因型和A等位基因为RA的危险因素.
- 在RA患者中观察到IL-6,miR-146a和MALAT1的表达水平升高,不管他们的SNP状态如何.
结论:
- 在IL-6,miR-146a和MALAT1中的SNP与埃及人患风湿性关节炎的倾向性增加有关.
- 这些发现表明,这些遗传变异和基因表达在该群体内的RA病变发生过程中可能发挥作用.
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