通过光谱和成像技术,通过冷大气压等离子体喷射阐明细菌失活机制
Sarthak Das1, Sarita Mohapatra1,2, Satyananda Kar1,3
1School of Interdisciplinary Research, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
冷大气压等离子喷流 (CAPJ) 通过破坏细胞形态和分子组成,有效地使大肠杆菌和金黄色杆菌等细菌失活. 这项研究揭示了增强绝育应用的关键失活路径.
科学领域:
- 等离子体物理学和化学等离子体物理学和化学
- 微生物学 微生物学
- 光谱学和显微镜学
背景情况:
- 冷大气压等离子喷流 (CAPJ) 是一种有前途的非热杀菌方法.
- 了解CAPJ对细菌无活化的特定机制对于优化其应用至关重要.
- (Ar) 血正在研究其在细菌不活化方面的潜力.
研究的目的:
- 为了阐明由 (Ar) 冷大气压等离子体喷流 (CAPJ) 排放引起的细菌失活路径.
- 描述Ar CAPJ对细菌细胞形态和分子组成的影响.
- 为了确定负责细菌失活的反应性物种.
主要方法:
- Ar CAPJ的电特性和反应性物种的表征.
- 使用富里埃变换红外 (FTIR),拉曼微光谱学和传输电子显微镜 (TEM) 评估细菌的化学组成和形态学.
- 对细菌减少的量化和对大肠杆菌和金黄色葡萄球菌的D值的确定.
主要成果:
- 在60秒和120秒内分别实现了大肠杆菌和金黄色杆菌的>6日志减少,D值低 (<20秒).
- FTIR和拉曼光谱显示了分子损伤,包括脂质过氧化和蛋白质氧化.
- TEM成像显示了由CAPJ反应性物种引起的细菌细胞的显著形态损伤.
- 识别了关键的气相反应性物种 (例如,Ar I,O I,OH•,NO+,NO2-,NO3-),这些物种有助于无活化.
结论:
- 通过诱导细胞形态和分子组成的显著变化,Ar CAPJ有效地使细菌失活.
- 该研究揭示了由Ar CAPJ产生的特定反应性物种驱动的潜在细菌失活机制.
- 研究结果为开发先进的基于等离子体的灭菌技术提供了洞察力.
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