利莫西拉ctobacillus 调节微生物群落以克服水解瓶,通过高效的一步联合生产H2和CH4克服水解瓶
Heng Wu1,2,3, Huaiwen Zhang1,2,3, Ruixiao Yan4
1College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|September 12, 2024
概括
乳酸细菌增强了单步生物气生产,显著增加了和甲产量. 这种方法可以提高无氧消化过程中的能量产生和利能力.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 通过无氧消化 (AD) 有效的 (H2) 和甲 (CH4) 的联合生产具有挑战性,通常需要单独的阶段.
- 乳酸细菌 (LAB) 可以增强水解,可能导致同时H2和CH4的积累.
研究的目的:
- 在AD期间研究LAB在H2和CH4的一步共产中的增强效果.
- 阐明 LAB 介导的生物气生产增强背后的机制.
主要方法:
- 使用的Limosilactobacillus菌株具有特定的基因 (bglB,xynB) 和酶 (蛋白酶,乙酸酶,[FeFe]酶).
- 作为模型基质使用了红纤维素草,酸和H2O.
- 在整个AD过程中 (0-24天) 分析了微生物社区的转变和酶活动.
主要成果:
- 在H2生产中实现了236.3倍的增长,在CH4生产中增加了7.1倍.
- 通过提高生物气产量,表现出显著的利能力 (469.39美元).
- 确定了LAB在最初6天的水解和挥发性脂肪酸生产中的作用,驱动性甲基生成.
- 从第7天开始,观察到向乙类甲基生成的转变,由富含的甲沙辛和增加的甲四甲诺氨酸化酶活性驱动.
- 与中国标准生物气厂相比,电力发电能力增加了333.33倍.
结论:
- Limosilactobacillus通过改善水解和挥发性脂肪酸积累,有效地增强了AD中的单步H2和CH4联合生产.
- 该研究阐明了驱动同时产生H2和CH4的微生物和酶机制以及随后的甲基生成转移.
- 通过LAB增强的AD为生物气生产提供了一种高效和有利可图的方法,具有显著增加的能源生产潜力.
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