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Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes
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采用Opal多重体免疫光学来表征小鼠肠道上皮细胞系谱的协议.

Anxo Martinez-Ordoñez1, Hiroto Kinoshita1, Tania Cid-Diaz1

  • 1Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

STAR protocols
|September 12, 2024
PubMed
概括

这项研究提出了一种使用Opal多重体免疫光 (OMI) 来表征小鼠肠道上皮细胞系谱的协议. 该方法优化了基因工程小鼠模型中多个目标的信号检测.

关键词:
癌症 癌症 癌症 癌症在现场混合化.显微镜的使用方法模型生物模型生物分子生物学分子生物学分子/化学探头 分子/化学探头干细胞是干细胞.

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科学领域:

  • 免疫组织化学和细胞生物学.
  • 胃肠道研究 胃肠道研究
  • 鼠标模型研究的研究.

背景情况:

  • 奥巴尔多重体免疫光 (OMI) 是分析组织中复杂生物过程的强大工具.
  • 在小鼠模型中表征肠上皮细胞系对于理解胃肠道疾病至关重要.
  • 使用OMI在肠道组织中同时检测多个点分子具有技术上的挑战.

研究的目的:

  • 通过使用OMI来描述小鼠肠道上皮细胞谱系的详细方案.
  • 优化OMI面板设计和信号检测,用于小鼠肠道组织.
  • 在这种情况下,提供一种用于对OMI图像进行定量评估的方法.

主要方法:

  • 为处理小鼠小肠和结肠组织制定协议.
  • 设计和优化OMI面板用于在小鼠肠道中多目标检测.
  • 对OMI数据的定量图像分析程序.

主要成果:

  • 建立了一个标准化协议,用于在小鼠肠道组织中应用OMI.
  • 优化的OMI面板能够对多个肠上皮细胞标记物的强大检测.
  • 定量评估方法允许对细胞谱系标记物的可靠评估.

结论:

  • 开发的OMI协议对于表征小鼠肠道上皮细胞谱系是有效的.
  • 这种方法增强了基因工程小鼠模型中复杂细胞过程的研究.
  • 该协议促进了肠道组织结构的详细空间和定量分析.