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Updated: Jun 13, 2025

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将针头变成禾:直接从它们的原生环境中培养复杂微生物基因组的文化独立放大

Olivia A Pilling1, Sesh A Sundararaman2, Dustin Brisson3

  • 1Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.

PLoS pathogens
|September 12, 2024
PubMed
概括

选择性全基因组放大 (SWGA) 能够从复杂样本中测序低丰度微生物. 这种技术有助于研究病原体动态和抗菌素耐药性,克服培养限制.

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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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